Iuliis Geoffry N. De, Rhiannon J. Newey, Bruce V.
Mobile Phone Radiation Induces Reactive Oxygen Species Production and DNA Damage in Human Spermatozoa In Vitro
PLoS One. 2009 Jul 31;4(7):e6446
Geoffry N. De Iuliis , Rhiannon J. Newey, Bruce V. King, R. John Aitken
ARC Centre of Excellence in Biotechnology and Development, Callaghan, New South Wales, Australia, 2 School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales, Australia, 3 School of Mathematical and Physical Sciences, The University of Newcastle, Callaghan, New South Wales, Australia
GMS 1800 MHz
Purified human spermatozoa were exposed to radio-frequency electromagnetic radiation (RF- EMR) tuned to 1.8 GHz and covering a range of specific absorption rates (SAR) from 0.4 W/kg to 27.5 W/kg. In step with increasing SAR, motility and vitality were significantly reduced after RF-EMR exposure, while the mitochondrial generation of reactive oxygen species and DNA fragmentation were significantly elevated (P<0.001). Furthermore, we also observed highly significant relationships between SAR, the oxidative DNA damage bio-marker, 8-OH-dG, and DNA fragmentation after RF-EMR exposure.
High quality spermatozoa selected in discontinuous Percoll gradients displayed a decline in both vitality and motility after exposure to RF-EMR in a dose- dependent manner. The control populations maintained an average vitality of 89%; however, significant reductions in vitality were observed at exposure levels as low as 1.0 W/kg (p<0.01) (Figure 2A). Similarly, the control populations maintained motilities at an average of 86% over the incubation period, however after exposure to RF-EMR at levels of 1.0 W/kg, motility was observed to significantly decrease to 68% (p<0.05) and decreased still further at higher SAR exposures (Figure 2B).
RF-EMR in both the power density and frequency range of mobile phones enhances mitochondrial reactive oxygen species generation by human spermatozoa, decreasing the motility and vitality of these cells while stimulating DNA base adduct formation and, ultimately DNA fragmentation. These findings have clear implications for the safety of extensive mobile phone use by males of reproductive age, potentially affecting both their fertility and the health and wellbeing of their offspring.